The US on Saturday warned Iran against retaliation after Israel attacked military bases in Tehran, Ilam and Khuzestan provinces.
This comes amid fears about a potential escaltion in the Middle East tensions as Israel continues to strike Iranian proxies in Lebanon.
Washington said Israeli strikes targeting Iran should 'complete' exchange of fire between enemies.
A senior Biden administration official said there will be consequences if Iran attacks Israel, adding that the US is fully prepared to once again defend any attack.
The official said the US has direct and indirect channels with Iran. "And they know our position on multiple issues," he added.
Iran said its air defence system has countered Israel's attacks successfully though "limited damage" was caused to some location. Iran has suspended all flights until further notice after the strikes were announced by Israel.
Israel has intensified its attack on Hezbollah, an Iran-backed Shia militant group, which has been supporting Hamas in the ongoing conflict.
Israel revealed its planes safely returned home after completing the attack.
