Fifteen-year-old prodigy Coco Gauff set up an Australian Open blockbuster with defending champion Naomi Osaka on Wednesday, as Serena Williams screamed and shouted her way into the third round.
On a day when men's seeds tumbled, Serbia's Novak Djokovic strode towards his eighth Melbourne title and soon-to-retire Caroline Wozniacki lived to fight another day.
American teenager Gauff, showing a tenacity which is becoming her trademark, clawed her way back from a set down to beat the experienced Sorana Cirstea 4-6, 6-3, 7-5.
Gauff also beat Williams on her Grand Slam debut last year at Wimbledon, and in another quirk, she faced Osaka in the US Open third round, going down meekly in straight sets.
Japan's Osaka, 22, beat China's Zheng Saisai 6-2, 6-4 but briefly lost her cool mid-match, hurling and kicking her racquet when she gave up a break in the second set.
Former world number one Wozniacki, playing her final tournament before retiring, came from behind in both sets for a 7-5, 7-5 win over Dayana Yastremska of Ukraine.
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Serena Williams shouted at the top of her voice during a testing second set against 70th-ranked Tamara Zidansek, before seeing off break points to win 6-2, 6-3. Williams can equal Margaret Court's record of 24 Grand Slams if she lifts the trophy.
Earlier, Australia's world number one Ashleigh Barty raced through 6-1, 6-4 against Polona Hercog, buoying hopes of a first home winner since 1978.
Djokovic also weathered the breeze, which followed heavy rain on Monday, and smog from bushfires last week, to beat Japanese wildcard Tatsuma Ito 6-1, 6-4, 6-2 in 95 minutes.
Greece's Stefanos Tsitsipas was handed a slice of fortune when Philipp Kohlschreiber withdrew injured.
In other results, Petra Kvitova, last year's runner-up, came through 7-5, 7-5 against Paula Badosa of Spain, and China's Zhang Shuai ousted American Caty McNally 6-2, 6-4.
Former US Open champion Marin Cilic won a five-setter against French seed Benoit Paire, and one-time semi-finalist Milos Raonic beat Chile's Christian Garin in straights.
Tennys Sandgren, engulfed in controversy over his political views and right-wing links during his run to the 2018 quarter-finals, took the biggest scalp so far when he ousted eighth seed Matteo Berrettini in five sets.
Sandgren's fellow American Tommy Paul also pulled off a five-set upset when he beat Bulgarian 18th seed Grigor Dimitrov 6-4, 7-6 (8/6), 3-6, 6-7 (3/7), 7-6 (10/3).
And Australia's John Millman further trimmed the number of high-ranked players when he ended the campaign of Polish 31st seed Hubert Hurkacz.
Among eight first-round matches held over from Tuesday, after rain wiped out half of Monday's schedule, Spain's Carla Suarez Navarro, also retiring this year, upset Belarusian 11th seed Aryna Sabalenka in straight sets.
